A T75 flask is a type of tissue culture flask commonly used in cell culture applications. The “T” in T75 stands for “tissue culture,” and the number 75 refers to the approximate growth area of the flask, which is 75 square centimeters (cm²). These flasks are specifically designed for the growth and maintenance of adherent and suspension cell lines.
T75 flasks are typically made of polystyrene and are sterilized to ensure aseptic conditions for cell culture. They have a rectangular shape with a flat surface for adherent cell growth and a vented cap that allows for gas exchange while minimizing the risk of contamination.
Key features of T75 flasks include:
- A large surface area (75 cm²) for cell growth, making them suitable for the expansion of cell lines and the production of larger cell populations.
- Graduated markings on the side of the flask to indicate the volume of media added, which helps ensure consistency and reproducibility across experiments.
- A vented or filter cap that maintains aseptic conditions within the flask while allowing for adequate gas exchange (oxygen and carbon dioxide).
- An angled neck, which facilitates the easy removal and addition of cell culture media without disturbing the adherent cells on the surface.
To use a T75 flask for cell culture, first, add the appropriate volume of sterile cell culture media to the flask (usually between 15 to 20 mL). Then, inoculate the flask with a cell suspension, making sure to distribute the cells evenly across the surface. Place the flask in a suitable incubator, maintaining the required temperature, humidity, and CO₂ concentration for your specific cell line. Monitor the growth and confluency of the cells regularly and passage them as needed.
