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Manipulate Genetically
Genetic manipulation, also known as genetic engineering, is the direct alteration of an organism’s genes using biotechnology. Genetic manipulation has been employed in various fields, including agriculture, medicine, and scientific research, to produce genetically modified organisms (GMOs) with desired traits or capabilities. There are several methods used for genetic manipulation: Genetic manipulation has the potential…
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Mesothelioma
Mesothelioma is a rare and aggressive form of cancer that primarily affects the mesothelium, a thin layer of specialized cells lining the body’s internal organs and cavities. The mesothelium produces a lubricating fluid that enables the smooth movement of organs against one another, such as the expansion and contraction of the lungs during breathing. The…
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Recombinant Plasmids
Recombinant plasmids are circular DNA molecules that are artificially created by combining DNA from different sources. They are commonly used in molecular biology, genetics, and biotechnology for various purposes, including gene cloning, gene expression studies, and the production of recombinant proteins. Recombinant plasmids are typically constructed using a process called “molecular cloning,” which involves the…
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Dissociation Agent
A dissociation agent is a substance used to separate cells from each other or from their extracellular matrix in cell culture or tissue samples. The dissociation process is essential for various research applications, such as passaging or subculturing cells, preparing single-cell suspensions for flow cytometry, or isolating specific cell types for further study. Dissociation agents…
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Confluency
Confluency refers to the percentage of the surface area in a cell culture vessel that is covered by adherent cells. In other words, it is a measure of how crowded or dense the cells are within the culture vessel. Confluency is a crucial parameter in cell culture, as it influences cell growth, morphology, behavior, and…
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Cells Undergo Genotypic
Cells undergo genotypic changes or alterations in their genetic makeup due to various factors, including mutations, chromosomal rearrangements, and gene amplification or deletion events. These genotypic changes can impact the cell’s phenotype, including morphology, behavior, and function, and can sometimes lead to diseases or abnormal cellular functions. Cells have various mechanisms in place to repair…
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Culture Flask
A culture flask is a type of laboratory vessel specifically designed for growing and maintaining cells in a controlled environment. Culture flasks are commonly used for adherent cell cultures, where cells require a surface to attach and grow, as well as suspension cell cultures, where cells grow freely in the liquid medium. Culture flasks come…
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Cell Suspension
A cell suspension refers to a liquid mixture containing individual cells that are uniformly distributed within the medium. Cell suspensions are commonly used in various laboratory and research settings, especially in cell culture, where cells are grown and maintained in a controlled environment. Cell suspensions can be derived from adherent cell cultures, tissue dissociation, or…
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Ratio Aliquot
In the context of cell culture and laboratory work, a ratio aliquot refers to dividing a solution or cell suspension into smaller, equal parts or aliquots based on a specific ratio. This process is often performed when diluting a solution, subculturing cells, or preparing samples for experiments that require equal concentrations or cell densities. For…
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Trypsin
Trypsin is a serine protease enzyme that plays a crucial role in the digestion of proteins. It is produced in the pancreas as an inactive precursor called trypsinogen, which is then secreted into the small intestine. Once in the small intestine, trypsinogen is activated to trypsin by the enzyme enteropeptidase (also called enterokinase). Trypsin functions…