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Clustering
Clustering is an unsupervised machine learning technique used to group data points based on their similarity or common features. The primary objective of clustering is to identify the underlying structure in the data, separating it into different groups or clusters. Each cluster consists of data points that are more similar to each other than to…
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Cell Detachment Procedure
The cell detachment procedure is a critical step in cell culture for subculturing, harvesting cells, or performing downstream experimental applications. The choice of detachment method depends on the cell type, culture conditions, and the specific experimental requirements. Here is a general procedure for enzymatic cell detachment using trypsin-EDTA, which is commonly used for adherent cell…
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Enzymatic Activity
Enzymatic activity refers to the ability of enzymes to catalyze biochemical reactions in living organisms. Enzymes are specialized proteins that serve as biological catalysts, accelerating the rate of chemical reactions without being consumed or altered in the process. They play crucial roles in various cellular processes, such as metabolism, signal transduction, and DNA replication. Enzymatic…
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Detachment of Cells
Detachment of cells refers to the process of releasing adherent cells from the surface of a culture vessel, such as a flask or dish, in order to subculture, harvest, or perform downstream experimental procedures. Detachment can be achieved through various methods, including enzymatic, mechanical, or chemical approaches, depending on the cell type, culture conditions, and…
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Inverted Microscope
An inverted microscope is a type of optical microscope that has its light source and condenser located above the specimen, with the objectives and turret below it. This design is the opposite of a conventional, or upright, microscope, where the light source and condenser are located below the specimen, and the objectives are above it.…
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T75 Flask
A T75 flask is a type of tissue culture flask commonly used in cell culture applications. The “T” in T75 stands for “tissue culture,” and the number 75 refers to the approximate growth area of the flask, which is 75 square centimeters (cm²). These flasks are specifically designed for the growth and maintenance of adherent…
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Dulbecco’s MEM
Dulbecco’s Modified Eagle’s Medium (DMEM) is a widely used cell culture medium formulated to support the growth of a variety of mammalian cells in vitro. It was developed by Renato Dulbecco and Marguerite Vogt in the late 1950s as a modification of the original Eagle’s Basal Medium, which was formulated by Harry Eagle. DMEM typically…
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Trypsin-EDTA
Trypsin-EDTA is a solution used in cell biology and tissue culture to facilitate the detachment of adherent cells from the surfaces of culture vessels. It is a mixture of two components: trypsin and ethylenediaminetetraacetic acid (EDTA). The combination of trypsin and EDTA effectively detaches cells from their culture vessel, allowing them to be harvested and…
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T75 Flask
A T75 flask, also known as a T-75 or 75 cm² tissue culture flask, is a standard cell culture vessel used to grow and maintain adherent and suspension cells in vitro. The “T” stands for tissue culture, and the number “75” refers to the growth surface area of the flask, which is approximately 75 square…
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Sterile 1x PBS
Sterile 1x Phosphate-Buffered Saline (PBS) is a commonly used buffer solution in cell biology, biochemistry, and molecular biology research. It consists of a balanced salt solution containing sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. PBS helps maintain the osmolarity and pH of the solution, which is crucial for maintaining cell integrity and function…